Manual Plant Cell Culture

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Hayta S, Bayraktar M, Baykan erel S, Gurel A Direct plant regeneration from different explants through micropropagation and determination of secondary metabolites in the critically endangered endemic Rhaponticoides mykalea.


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Bot Stud — Google Scholar. Pharmacogn Mag — J Pharm Bioallied Sci — Jamwal K, Bhattacharya S, Puri S Plant growth regulator mediated consequences of secondary metabolites in medicinal plants.

Tasty superfood from plant cell cultures

J Med Plant Res — Meyer hairy roots. Karuppusamy S A review on trends in production of secondary metabolites from higher plants by in vitro tissue, organ and cell cultures. Physiol Mol Biol Plants — Int J Food Sci Tech — Biotechnol Lett — Springer, New York, pp 15— Klee H, Horsch R, Rogers S Agrobacterium-mediated plant transformation and its further applications to plant biology. Annu rev Plant Physiol — Kodym A, Hollenthoner S, Zapata-Arias FJ Cost reduction in the micropropagation of banana by using tubular skylights as source for natural lighting.

In vitro Cell Dev Biol Plant — Mol Pharm — Biomolecules — Lajayer BA, Ghorbanpour M, Nikabadi S Heavy metals in contaminated enviroment: destiny of secondary metabolite biosynthesis, oxidative status and phytoextraction in medicinal plants. Ecotoxicol Environ Saf — PLoS One 8:e Larkin PJ, Scowcroft WR Somaclonal variation: a novel source of variability from cell cultures for plant improvement. Theor Appl Genet — Nat Biotechnol — Leibniz Gemeinschaft Catalogue of plant cell lines.

Plant Physiol — Lubbe A, Verpoorte R Cultivation of medicinal and aromatic plants for specialty industrial materials. Ind Crop Prod — Maleki M, Ghorbanpour M, Kariman K Physiological and antioxidative responses of medicinal plants exposed to heavy metals stress. Plant Gene — Martin M Predatory prokaryotes: an emerging research opportunity.

OmniaScience, Barcelona, pp — Mulabagal V, Tsay H-S Plant cell cultures—an alternative and efficient source for the production of biologically important secondary metabolites. Muranaka T, Ohkawa H, Yamada Y Continuous production of scopolamine by a culture of Duboisia leichhardtii hairy root clone in a bioreactor system. Murthy HN, Lee EJ, Paek KY Production of secondary metabolites from cell and organ cultures: strategies and approaches for biomass improvement and metabolite accumulation.

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Am J Plant Physiol — Shilpa K, Varun K, Lakshmi BS An alternate method of natural drug production: eliciting secondary metabolite production using plant cell culture. J Plant Sci — Backer ex K. Heyne using DNA-based markers. Sivanandhan G, Selvaraj N, Ganapathi A, Manickavasagam M Enhanced biosynthesis of withanolides by elicitation and precursor feeding in cell suspension culture of Withania somnifera L.

Dunal in Shake-Flask culture and bioreactor. PLoS One 9:e Smetanska I Production of secondary metabolites using plant cell cultures. Adv Biochem Eng Biotechnol — Srivastava S, Srivastava AK Hairy root culture for mass-production of high-value secondary metabolites. Crit Rev Biotechnol — Srivastava S, Srivastava AK Effect of elicitors and precursors on Azadirachtin production in hairy root culture of Azadirachta indica. Appl Biochem Biotechnol — In: Yang S-T ed Bioprocessing for value-added products from renewable resources.

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Plant cell cultures for the production of recombinant proteins | Nature Biotechnology

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  • Table of contents.
  • Plant cell culture technology in the cosmetics and food industries: current state and future trends;
  • Industry Insights?
  • INTRODUCTION;

Transgen Res — Plant J — Hairy root cultures and evaluation of factors affecting growth and xanthone production. Voytas DF Plant genome engineering with sequence-specificnucleases. Annu Rev Plant Biol — Voytas DF, Gao C Precision genome engineering and agriculture: oportunities and regulatory challenges. PLoS Biol —6. Phytochemistry Google Scholar. J Photochem Photobiol B — Single cells, plant cells without cell walls protoplasts , pieces of leaves, stems or roots can often be used to generate a new plant on culture media given the required nutrients and plant hormones.

Preparation of plant tissue for tissue culture is performed under aseptic conditions under HEPA filtered air provided by a laminar flow cabinet.

1. Introduction

Thereafter, the tissue is grown in sterile containers, such as petri dishes or flasks in a growth room with controlled temperature and light intensity. Living plant materials from the environment are naturally contaminated on their surfaces and sometimes interiors with microorganisms , so their surfaces are sterilized in chemical solutions usually alcohol and sodium or calcium hypochlorite [1] before suitable samples known as explants are taken.

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The sterile explants are then usually placed on the surface of a sterile solid culture medium, but are sometimes placed directly into a sterile liquid medium, particularly when cell suspension cultures are desired. Solid and liquid media are generally composed of inorganic salts plus a few organic nutrients, vitamins and plant hormones.

Solid media are prepared from liquid media with the addition of a gelling agent, usually purified agar. The composition of the medium, particularly the plant hormones and the nitrogen source nitrate versus ammonium salts or amino acids have profound effects on the morphology of the tissues that grow from the initial explant. For example, an excess of auxin will often result in a proliferation of roots, while an excess of cytokinin may yield shoots. A balance of both auxin and cytokinin will often produce an unorganised growth of cells, or callus , but the morphology of the outgrowth will depend on the plant species as well as the medium composition.

As cultures grow, pieces are typically sliced off and subcultured onto new media to allow for growth or to alter the morphology of the culture. The skill and experience of the tissue culturist are important in judging which pieces to culture and which to discard. As shoots emerge from a culture, they may be sliced off and rooted with auxin to produce plantlets which, when mature, can be transferred to potting soil for further growth in the greenhouse as normal plants.

The specific differences in the regeneration potential of different organs and explants have various explanations. The significant factors include differences in the stage of the cells in the cell cycle , the availability of or ability to transport endogenous growth regulators, and the metabolic capabilities of the cells. The most commonly used tissue explants are the meristematic ends of the plants like the stem tip, axillary bud tip and root tip.


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These tissues have high rates of cell division and either concentrate or produce required growth regulating substances including auxins and cytokinins. Shoot regeneration efficiency in tissue culture is usually a quantitative trait that often varies between plant species and within a plant species among subspecies, varieties, cultivars , or ecotypes.

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Therefore, tissue culture regeneration can become complicated especially when many regeneration procedures have to be developed for different genotypes within the same species. The three common pathways of plant tissue culture regeneration are propagation from preexisting meristems shoot culture or nodal culture , organogenesis and non-zygotic embryogenesis.

The propagation of shoots or nodal segments is usually performed in four stages for mass production of plantlets through in vitro vegetative multiplication but organogenesis is a common method of micropropagation that involves tissue regeneration of adventitious organs or axillary buds directly or indirectly from the explants. Non-zygotic embryogenesis is a noteworthy developmental pathway that is highly comparable to that of zygotic embryos and it is an important pathway for producing somaclonal variants, developing artificial seeds, and synthesizing metabolites.

Due to the single cell origin of non-zygotic embryos, they are preferred in several regeneration systems for micropropagation, ploidy manipulation, gene transfer, and synthetic seed production. Nonetheless, tissue regeneration via organogenesis has also proved to be advantageous for studying regulatory mechanisms of plant development.